Amplification from a single cell reach threshold at around 10 cycles 1 cycle 5 minutes.
Single cell genomiphi dna amplification kit.
Single cell genomiphi dna amplification kit combines the unique capabilities of phi29 dna polymerase with an optimized formulation to amplify genomic dna from 1 1000 cells without background interference.
Whole genome amplification of genomic dna from as little as a single cell.
Single cell genomiphi dna amplification kit has been optimized to wholly amplify genomic dna from as little as a single cell generating micrograms of high quality dna for use in downstream applications.
Single cell genomiphi dna amplification kit is sensitive up to amplification of 1 fg of gdna.
Figure 3 shows the real time amplification of single female human cells with a ntc.
At very low levels of input dna successful amplification relies on reagent quality.